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KMID : 0614020000160010025
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Journal of Pharmaceutical Sciences (C.N.U.)
2000 Volume.16 No. 1 p.25 ~ p.31
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Analysis of β-Blocker Enantiomers by Capillary Electrophoresis
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Lim Hwan-Mi
Linh Pharn-Duan
Kang Jong-Seong
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Abstract
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The effects of type of chiral selectors, pH and concentration of electrolyte on the resolution of the enantiomers of β-blockers by capillary electrophoresis were examined and the analysis of β-blocker enantiomer was attempted using various chiral selectors, such as carboxymethyl-, methyl-, succinyl, hydroxypropyl and dimethyl-β-cyclodextrin. Among the used chiral selectors, carboxymethyl-β-cyclodextrin (CM-β-CD) was the best for the separation of β-blockers. The optimum separation of the enantiomers of β-blocker was achieved on fused silica capillary (75 um i.d.×47 cm, 40 cm to detector) with phosphate buffer (pH 6, 70 mM) containing 10 mM of CM-β-CD as electrolyte at 10 KV running voltage. Injection was hydrodynamically for 3 psi×s and detection was carried out at UV 210nm.
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KEYWORD
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β-Blocker, enantiomer, β-cyclodextrin, capillary electrophoresis
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